This laboratory book from CBS Publishers & Distributors Pvt Ltd is an English medium (अंग्रेजी माध्यम) book on In Vitro Experimental Pharmacology, written by Asmita Gajbhiye Patil, Shailendra Patil, Shweta Mishra and Debashree Das, prepared for postgraduate students of pharmacology and pharmacy (M.Pharm, MD Pharmacology), research scholars and laboratory workers, and also useful to undergraduate pharmacy and medical students being introduced to experimental pharmacology. As regulatory and ethical practice moves screening away from whole-animal work, the book sets out the in vitro alternatives that have replaced it — each experiment given as a working protocol with its principle, requirements, reagents, stepwise procedure, observations, calculations and interpretation (व्याख्या), written so that a student can carry the book to the bench and perform the assay. The book covers good laboratory practice and handling of in vitro apparatus, preparation of buffers and physiological salt solutions, isolated tissue preparations and organ bath techniques with dose–response curves, agonist and antagonist studies and the determination of pA2, cell culture basics and maintenance of cell lines, cytotoxicity and cell viability assays, enzyme inhibition assays, antioxidant and free radical scavenging methods, anti-inflammatory, antidiabetic, antimicrobial and anticancer in vitro screening models, receptor binding studies, and the statistical treatment and reporting of experimental data. Its distinguishing feature is this protocol-first arrangement — reproducible methods presented in the sequence of an actual laboratory session, supported by diagrams, tables and worked calculations. All content, terminology and explanations in this book are presented in the English language only. Key Features: English medium book — complete content, terminology and explanations in English Written by Asmita Gajbhiye Patil, Shailendra Patil, Shweta Mishra and Debashree Das, published by CBS Publishers & Distributors Pvt Ltd Dedicated laboratory manual on in vitro methods in experimental pharmacology Each experiment given with principle, requirements, reagents, procedure, observations and interpretation In vitro alternatives to animal experimentation, in line with current ethical and regulatory practice Diagrams, tables and worked calculations for laboratory use Written for M.Pharm, MD Pharmacology, research scholars and laboratory workers Also useful for undergraduate pharmacy and medical students Good laboratory practice, safety and handling of in vitro apparatus Preparation of buffers and physiological salt solutions Isolated tissue preparations and organ bath techniques Dose–response curves, agonist and antagonist studies and determination of pA2 Cell culture basics, aseptic technique and maintenance of cell lines Cytotoxicity and cell viability assays Enzyme inhibition assays Antioxidant and free radical scavenging methods In vitro anti-inflammatory and antidiabetic screening models In vitro antimicrobial and anticancer screening models Receptor binding studies Statistical treatment, documentation and reporting of experimental data Useful for postgraduate practical examinations, dissertation and research work Pharmacology एवं Pharmacy के स्नातकोत्तर विद्यार्थियों, शोधार्थियों तथा प्रयोगशाला कर्मियों के लिए CBS Publishers & Distributors Pvt Ltd द्वारा प्रकाशित यह "In Vitro Experimental Pharmacology" पुस्तक Asmita Gajbhiye Patil, Shailendra Patil, Shweta Mishra एवं Debashree Das द्वारा लिखी गई है; यह स्नातक स्तर के pharmacy एवं medical विद्यार्थियों के लिए भी उपयोगी है। इसमें good laboratory practice, buffer एवं physiological salt solutions की तैयारी, isolated tissue एवं organ bath तकनीक, dose–response curves, cell culture, cytotoxicity एवं cell viability assays, enzyme inhibition, antioxidant, anti-inflammatory, antidiabetic, antimicrobial व anticancer in vitro screening models तथा प्रायोगिक आँकड़ों के सांख्यिकीय विश्लेषण को principle, procedure, observations एवं interpretation सहित समझाया गया है। सम्पूर्ण पुस्तक अंग्रेजी माध्यम (English medium) में है, इसमें हिंदी माध्यम की सामग्री सम्मिलित नहीं है।